Wizard Minicolumns from Promega

Product Specs
ItemWizard Minicolumns
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA7211
Quantity250 each
SourceReaction, Gel
Nucleic AcidDNA Fragment, PCR Product

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Lower viral evolutionary pressure under stable versus fluctuating conditions in subzero Arctic brinesMicrobiomeAugust 7, 2023Zhi-Ping Zhong, Dean Vik, Josephine Z. Rapp, Olivier Zablocki, Heather Maughan, Ben Temperton, Jody W. Deming, Matthew B. SullivanDNA was extracted using Wizard® PCR Preps DNA Purification Resin and Minicolumns (Cat. No. A7181 and A7211, respectively; Promega, USA) [70]. Short- and long-read virome sequencing Following DNA extraction,

(2) Freshwater and anadromous fishing in Ice Age BeringiaScience AdvancesJune 2, 2023Ben A. Potter, Carrin M. Halffman, Holly J. McKinney, Joshua D. Reuther, Bruce P. Finney, François B. Lanoë, J. Andrés López, Charles E. Holmes, Erica Palmer, Marie Capps, et al.guanidine HCl were added. The tubes were vortexed multiple times over approximately a 2 min period. Promega Wizard minicolumns were attached to 3-ml Luer-Lok syringe barrels (minus the plunger) and placed on a vacuum manifold.

(3) Glacier ice archives nearly 15,000-year-old microbes and phagesMicrobiomeDecember 1, 2021Zhi-Ping Zhong et al.was then extracted using Wizard® PCR Preps DNA Purification Resin and Minicolumns (Cat. No. A7181 and A7211, respectively; Promega, USA) [45]. Viral abundance, calculated prior to DNA extraction, was obtained

(4) Polinton-like viruses are abundant in aquatic ecosystemsMicrobiomeDecember 1, 2021Christopher M. Bellas, Ruben SommarugaCentrifugal Filter Units (Merck-Millipore UFC910024). DNA was extracted from the mixture using 4 × Promega Wizard® Minicolumns per replicate to extract DNA with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin

(5) Seasonal and diel patterns of abundance and activity of viruses in the Red SeaProceedings of the National Academy of SciencesNovember 24, 2020Gur Hevroni, JosÃ© Flores-Uribe, Oded BÃ©jÃ , Alon PhilosofCsCl gradient followed by DNase treatment as described in ref. 68. DNA extraction was performed using Wizard minicolumns (Promega) as described in ref. 69. The metatranscriptome fraction was collected onto a 142-mm 0.22-µm

(6) Ancient Beringian paleodiets revealed through multiproxy stable isotope analysesScience AdvancesSeptember 4, 2020Carrin M. Halffman, Ben A. Potter, Holly J. McKinney, Takumi Tsutaya, Bruce P. Finney, Brian M. Kemp, Eric J. Bartelink, Matthew J. Wooller, Michael Buckley, Casey T. Clark, et al.guanidine HCl were added. The tubes were vortexed multiple times over approximately a 2-min period. Promega Wizard minicolumns were attached to 3 ml of Luer-Lok syringe barrels (minus the plunger) and placed on a vacuum manifold.

(7) Viral Ecogenomics of Arctic Cryopeg Brine and Sea IcemSystemsJune 30, 2020Zhi-Ping Zhong et al.2a01:4f9:3a:1a85::2. MATERIALS AND METHODS using Wizard PCR Prep DNA puriﬁcation resin and minicolumns (no. A7181 and A7211, respectively; Promega, USA) (34). The DNA libraries were prepared using the Accel-NGS 1S Plus DNA library kit

(8) Subduing the influence of PCR inhibitors on amplifying aged, degraded, and low copy number DNA: PCR enhancer cocktail-p and rescue PCRPLOS ONEJune 16, 2020Brian M. Kemp, Brittany Bingham, Ryan Frome, Marie Labonte, Erica Palmer, Ella S. Parsons, Kenneth W. Gobalet, Jeffrey RosenthalHCl were added. The tubes were vortexed multiple times over approximately a 2 minute period. Promega Wizard minicolumns were attached to 3 mL luer-lok syringe barrels (minus the plunger) and placed on a vacuum manifold.

(9) Towards optimized viral metagenomes for double-stranded and single-stranded DNA viruses from challenging soilsPeerJJuly 4, 2019Gareth Trubl, Simon Roux, Natalie Solonenko, Yueh-Fen Li, Benjamin Bolduc, Josué Rodríguez-Ramos, Emiley A. Eloe-Fadrosh, Virginia I. Rich, Matthew B. Sullivanvolume) using one of three methods: Wizard mini columns (Wizard; Promega, Madison, WI, products A7181 and A7211), cetyl trimethylammonium bromide (CTAB; Porebski, Bailey & Baum, 1997), or modified DNeasy PowerSoil

(10) Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 InfectionAntibiotics (Basel)November 16, 2018Cristina Howard-Varona et al.mixed by inversion. The samples were put into a syringe and pushed through a Wizzard minicolumn (Cat. #A7211, Promega Corporation, Madison, WI, USA), followed by the addition of 2 mL 80% isopropanol pushed through

(11) Soil Viruses Are Underexplored Players in Ecosystem Carbon ProcessingmSystemsOctober 30, 2018Gareth Trubl et al.reference 50). The viral DNA was extracted using Wizard columns (Promega, Madison, WI; products A7181 and A7211), cleaned up with AMPure beads (Beckman Coulter, September/October 2018 Volume 3 Issue 5 e00076-18 msystems.asm.org

(12) Host-linked soil viral ecology along a permafrost thaw gradientNature MicrobiologyAugust 1, 2018Joanne B. Emerson et al.viral DNA was extracted from each of the seven subsamples, using Wizard columns and resin (A7181 and A7211, Promega), cleaned with AMPure beads (A63881, Beckman Coulter) and concentrated with Zymo DNA Clean

(13) Allelic effects on starch structure and properties of six starch biosynthetic genes in a rice recombinant inbred line populationRice (N Y)December 1, 2015Jixun Luo, Stephen A Jobling, Anthony Millar, Matthew K Morell, Zhongyi LiAfter the incubation, the samples were boiled in a urea solution (6 mM) for 1 min and filtered through Wizard miniColumns (Promega). Determination of the chain length distribution (CLD) of amylopectin was conducted by fluorescence-activated

(14) Ocean plankton. Patterns and ecological drivers of ocean viral communitiesScienceMay 22, 2015Jennifer R. Brum et al.ratio of 0.5 mL sample to 1 mL resin, and eluted with TE buffer (10 mM Tris, pH 7.5, 1 mM EDTA) using Wizard Minicolumns. Extracted DNA was Covaris-sheared and size selected to 160–180 bp, followed by amplification and ligation

(15) Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean SeaPLOS ONEJanuary 14, 2015Elena Lara, Karin Holmfeldt, Natalie Solonenko, Elisabet Laia Sà, J. Cesar Ignacio-Espinoza, Francisco M. Cornejo-Castillo, Nathan C. Verberkmoes, Dolors Vaqué, Matthew B. Sullivan, Silvia G. Acinasand mixed gently by inverting the tube. The mixture was loaded onto a mini-column (Promega, product A7211 Madison WI) through a 5 ml syringe attached to the column, pushing the mixture through with the syringe

(16) A Problem of Species Determination Using Housekeeping Genes in the Streptococcus anginosus GroupJuntendo Medical JournalJanuary 1, 2015TOMONORI MIZUTANI, SHIGEKAZU IGUCHI, KEIICHI HIRAMATSU, KEN KIKUCHIalso obtained by using corresponding primer sets listed in Table-1). The amplicons were purified using Wizard minicolumns (Promega, Madison, WI). The gene fragments were sequenced on both strands using the same primers and

(17) Clonality and Anatomic Distribution on the Skin of Antibiotic Resistant and Sensitive Propionibacterium acnesActa Dermato VenereologicaMarch 1, 2014Hans B Lomholt, Mogens KilianInstitutet, Stockholm, Sweden, served as positive control. Purification of PCR amplicons was performed on Wizard Minicolumns (Promega, Madison, Wis.). Sequencing of the amplified fragments in both directions was achieved with

(18) Sequencing platform and library preparation choices impact viral metagenomesBMC GenomicsDecember 1, 2013Sergei A Solonenko, J César Ignacio-Espinoza, Adriana Alberti, Corinne Cruaud, Steven Hallam, Kostas Konstantinidis, Gene Tyson, Patrick Wincker, Matthew B SullivanSYBR-stained virus particles. DNA was extracted using the Wizard Prep DNA Purification system (Promega, cat# A7211 and A7181). DNA Source for TARA metagenomes 20—60L seawater was collected and filtered for two TARA Oceans

(19) Activity-dependent phosphorylation of MeCP2 threonine 308 regulates interaction with NCoRNatureJuly 18, 2013Ebert DH, Gabel HW, Robinson ND, Kastan NR, Hu LS, Cohen S, Navarro AJ, Lyst MJ, Ekiert R, Bird AP, et al.spurious phosphorylation events following cell lysis. Lysates were boiled for 10 minutes, passed through Wizard Minicolumns (Promega) to remove larger molecules and insoluble material, and resolved by 8% SDS-PAGE gels, normalized

(20) Ancient DNA Analysis of Mid-Holocene Individuals from the Northwest Coast of North America Reveals Different Evolutionary Paths for MitogenomesPLoS ONEJuly 3, 2013Yinqiu Cui, John Lindo, Cris E. Hughes, Jesse W. Johnson, Alvaro G. Hernandez, Brian M. Kemp, Jian Ma, Ryan Cunningham, Barbara Petzelt, Joycellyn Mitchell, David Archer, Jerome S. Cybulski, Ripan S. Malhitubes were vortexed numerous times over a 2-minute period. These mixtures were pulled across Promega Wizard® Minicolumns using Luer-Lok syringes and a vacuum manifold. The silica pellets were rinsed by pulling 3 mL of 80%

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